Instrumental Method Of Analysis Notes | B Pharmacy 7th Semester Imperfect Pharmacy Notes

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Imperfect Pharmacy
B Pharmacy
Notes

 7th Semester 

INSTRUMENTAL METHOD OF ANALYSIS

Unit 1

UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectorsPhoto tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications – Spectrophotometric titrations, Single component and multi component analysis

OPEN UNIT 1

Unit 2

IR Spectroscopy
Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations

Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications


Flame Photometry
Principle, interferences, instrumentation and applications


Atomic absorption spectroscopy
Principle, interferences, instrumentation and applications


Nepheloturbidometry
Principle, instrumentation and applications

OPEN UNIT 2 

Unit 3

Introduction to chromatography

Adsorption and partition column chromatography
Methodology, advantages, disadvantages and applications.


Thin layer chromatography
Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.


Paper chromatography
Introduction, methodology, development techniques, advantages, disadvantages and applications


Electrophoresis
Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications

OPEN UNIT 3

Unit 4

Gas chromatography
Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications


High performance liquid chromatography (HPLC)
Introduction, theory, instrumentation, advantages and applications.

OPEN UNIT 4

Unit 5

Ion exchange chromatography
Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications


Gel chromatography
Introduction, theory, instrumentation and applications


Affinity chromatography
Introduction, theory, instrumentation and applications

OPEN UNIT 5
 

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